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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: A Single Glycan at the 99-Loop of Human Kallikrein-related Peptidase 2 Regulates Activation and Enzymatic Activity
doi: 10.1074/jbc.m115.691097
Figure Lengend Snippet: FIGURE 5. Comparative degradation rates and fragmentation patterns of human fibronectin in the presence of KLK2e (lanes 2–6) glyco-KLK2 (lanes 7–11) as determined by SDS-PAGE. Aliquots were taken at represen- tative intervals and analyzed by SDS-PAGE followed by Coomassie Blue stain- ing. Fibronectin alone is stable at 37 °C for at least 24 h. Overall, the break- downbyglyco-KLK2appearsmoreefficientandresultsinsomeuniquebands after 24-h incubation, as indicated by arrows.
Article Snippet: For
Techniques: SDS Page, Staining, Incubation
Journal: Parasites & Vectors
Article Title: Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis?
doi: 10.1186/s13071-015-0799-0
Figure Lengend Snippet: Type I Collagen degradation assay measured in culture supernatants from canine endothelial (A) and smooth muscle cells (B) untreated or treated with 1 μg/ml of DiES + 10 μg/ml of PLG, 1 μg/ml of DiES, 10 μg/ml of PLG, or with 1 μg/ml of DiES + 10 μg/ml of PLG + 50 mM of the εACA. The results were expressed as percentage of the Type I Collagen concentration in the culture supernatant from negative control cells (100%). Each point is the mean ± SD from three independent experiments. The asterisk (*) designates significant (p < 0.05) differences between DiES + PLG treatment and control groups.
Article Snippet: After incubation overnight at 4°C, wells were blocked with 1% BSA in PBS and incubated with a
Techniques: Degradation Assay, Concentration Assay, Negative Control